Bioinformatics Analysis and Validation of the Expressed Sequences Tag in Human Colorectal Adenocarcinoma
نویسندگان
چکیده
BACKGROUND This study was to investigate some new pathological genes in colorectal adenocarcinoma of human. METHODS Human colorectal adenocarcinoma tissues and normal colorectal tissues were taken and suppression subtractive hybridization (SSH) and cDNA microarray techniques were employed. From differentially expressed 86 expressed sequence tags (EST), 10 EST of the SSH were selected as seed sequence for bioinformatics analyses, semi-quantitative RT-PCR and PCR-sequencing. Each lane of semi-quantitative RT-PCR was analyzed by Q1 software. RESULTS Among these 10 EST, it has been found that ES274070, ES274071, ES274076 and ES274081 may play role in the onset of colorectal adenocarcinoma in human. CONCLUSIONS The ES274070, ES274071, ES274076 and ES274081 are related to the onset of human colorectal adenocarcinoma.
منابع مشابه
P-215: Discovery of A Novel APA Variant of A Human Potential Gene Based on Expressed Sequenced Tags Analysis
Background: Expressed sequence tags (ESTs) are sequences of cDNA fragments prepared from different tissue sources. There are over one million of these sequences in the publicly available database, and these sequences are believed to represent more than half of all human genes. The ESTs belong to different cDNA libraries, was prepared from one particular cell type, organ, or tumor. Therefore, th...
متن کاملComputational Identification of Micro RNAs and Their Transcript Target(s) in Field Mustard (Brassica rapa L.)
Background: Micro RNAs (miRNAs) are a pivotal part of non-protein-coding endogenous small RNA molecules that regulate the genes involved in plant growth and development, and respond to biotic and abiotic environmental stresses posttranscriptionally.Objective: In the present study, we report the results of a systemic search for identifi cation of new miRNAs in B. rapa using homology-based ...
متن کاملGene Regulation Network Based Analysis Associated with TGF-beta Stimulation in Lung Adenocarcinoma Cells
Background: Transforming growth factor (TGF)-β is over-expressed in a wide variety of cancers such as lung adenocarcinoma. TGF-β plays a major role in cancer progression through regulating cancer cell proliferation and remodeling of the tumor micro-environment. However, it is still a great challenge to explain the phenotypic effects caused by TGF-β stimulation and the effect of TGF-β stimulatio...
متن کاملAnalysis of a novel protein in human colorectal adenocarcinoma.
Colorectal adenocarcinoma (CRC) is the third most common type of cancer worldwide with a low 5‑year survival rate. The present study aimed to investigate the structure and function of a novel protein identified from human colorectal adenocarcinoma (CRC). A differentially expressed sequence tag (GenBank accession number, ES274081) was collected from GenBank. Bioinformatics tools were employed to...
متن کاملEffect of citric acid on p53 and p21 genes expression of the human colorectal Adenocarcinoma cell line
Background & Aim: Despite the advances in treatment of colorectal adenocarcinoma patients continue to have low survival. Citric acid is considered as a physiological inhibitor of enzymes involved in glycolysis pathway to remove cancer cells. The p53 and p21 gene products are involved in cell removal due to their pro-apoptotic properties. In this study, the effect of citric acid on p53 and p21 g...
متن کامل